Serological heterogeneity among fimbrial antigens causing mannose-resistant hemagglutination by uropathogenic Escherichia coli

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Serological Studies of O Antigens of Escherichia Coli by Means of the Hemagglutination Test.

Kunin, Calvin M. (University of Virginia, Charlottesville) and Mary V. Beard. Serological studies of O antigens of Escherichia coli by means of the hemagglutination test. J. Bacteriol. 85:541-548. 1963.-The serological interrelationships among most of the known O antigens of Escherichia coli were studied by the hemagglutination test. Good agreement with the bacterial agglutination test was foun...

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The role of mannose sensitive and mannose resistant pili of Escherichia coli in urinary tract infection

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Cloning of the chromosomal determinants encoding hemolysin production and mannose-resistant hemagglutination in Escherichia coli.

We have cloned the chromosomal hemolysin determinants from Escherichia coli strains belonging to the four O-serotypes O4, O6, O18, and O75. The hemolysin-producing clones were isolated from gene banks of these strains which were constructed by inserting partial Sau3A fragments of chromosomal DNA into the cosmid pJC74. The hemolytic cosmid clones were relatively stable. The inserts were further ...

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Oral vaccination of rats with live avirulent Salmonella derivatives expressing adhesive fimbrial antigens of uropathogenic Escherichia coli.

The avirulent Salmonella typhimurium F885 was transformed with a plasmid carrying the cloned S fimbriae genes of a uropathogenic Escherichia coli. The resulting transformant (F885-1) produced efficiently E. coli S fimbriae and was used for live oral vaccination of rats. For comparison rats were immunized subcutaneously with isolated S fimbriae. Both routes of vaccination resulted in a significa...

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Mannose-resistant hemagglutination, enzyme-linked immunosorbent assay, and immune electron microscopy for detection of K99 fimbrial antigen in Escherichia coli from calves.

Mannose-resistant hemagglutination (MRHA) was evaluated for identification of Escherichia coli with K99 fimbriae. The sensitivity and specificity of MRHA, relative to the enzyme-linked immunosorbent assay, were 21 and 79%, respectively. Disagreement between the tests may have been due in part to separation of pili from the cells, with resultant enzyme-linked immunosorbent assay-positive, MRHA-n...

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ژورنال

عنوان ژورنال: Infection and Immunity

سال: 1982

ISSN: 0019-9567,1098-5522

DOI: 10.1128/iai.35.2.745-748.1982